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ECIS System
Quantifying Cell Behavior


 

The ECIS is a turnkey system that provides researchers with an advanced, automated, non-invasive means to monitor cell behavior in real-time and without the use of labels.

ECIS measures the complex impedance of small 250-micrometer diameter electrodes used as substrates for cell growth. As cells grow on the electrode they constrict current flow altering the impedance. The ECIS reliably monitors up to 16 or 96 tissue culture wells. ECIS interprets the complex impedance as both resistance and capacitance and can report these values as well as the simple impedance.

Using a mathematical model the system can report time courses changes in the barrier function (permeability) of confluent cell layers as well as membrane capacitance and a term (alpha) related to the close contacts between the cell’s basal membrane and substrate. Other instruments that measure TER are designed for tight epithelial layers. ECIS is not limited to these layers and has proved particularly useful for studying endothelial cell layers with continuously and in real time.

The ECIS consists of a system controller, 16 and/or 96 well station, arrays, computer; integrated software runs on XP, Vista or Mac OS. Accessories include an optional flow module for specialized cell applications under flow conditions as well as an elevated field module to carry out automated wound-healing and electroporation experiments.


Applications

 

Wound-Healing - Cell Migration - Signal Transduction - Electroporation - Extravasation - Viral Cytopathic effects - Cells Under Flow

Proliferation - Angiogenesis - Cytotoxicity - Motility - Apoptosis - Chemotaxis - Barrier Function - Permeability - Cell-Cell Junctions

ECM Interactions

ECIS measures the change in impedance of a small electrode to AC current flow. The heart of the measurement is a specialized slide that has 8 or 96 individual wells for cell culturing. The base of the device has an array of gold film electrodes that connect to the ECIS electronics to each of the wells.

This technique is a patented technology known as ECIS, an acronym for Electric Cell-substrate Impedance Sensing. Cell densities ranging from a heavy confluent layer to very sparse layers can be measured with this approach. The size of the electrodes restricts the maximum number of anchored cells that can be observed from 100 to 1000 cells (dependent upon the type of electrode array used in the instrument), but even a single isolated cell results in impedance changes that can be monitored.

Two ECIS models has been designed with different features, the ECIS Z and the ECIS Z Teta. For more information, to download brochures and pubblications please visit the web page of Applied Biophisics Inc. following the link below listed.

 
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